To reconstruct biomimetic cell-dense tissues, one promising approach is through the use of a building-up process with cellular building blocks such as cell sheets. The manipulation and transplantation of such cellular building blocks rely on innovative strategies to noninvasively cleave cell-to-culture substrate connections while preserving cell-to-cell connections. In addition, it is necessary to fabricate thick tissues preliminarily in vitro and harvest them quickly for use in a transplant. In this study, we fabricate a transplantable thick cell sheet by employing the electrochemical detachment of cells from a membrane culture substrate.